Characterization of the proteinaceous organic matrix associated with carbonate mineral formed in the marine fish intestine

Tuesday, October 10, 2017 -
4:00pm to 5:00pm
Biotechnology Center, 425 Henry Mall

Speaker Name: 

Kevin Schauer, PhD

Speaker Institution: 

CIBM Postdoctoral Fellow, Genome Center of WI, University of Wisconsin-Madison




Marine fish live in a hyperosmotic environment and therefore must constantly counteract passive water loss and ion gain. To remain hydrated, marine teleosts (bony fish) must extract water from the only external water source available – seawater. Processing of imbibed seawater is completed in the highly specialized gastrointestinal tract, where inorganic CaCO3 is precipitated in the intestine, lowering luminal osmotic pressure, and aiding in fluid absorption. This precipitation reaction is crucial for the survival of marine fish (the largest vertebrate group on the planet) and is attributed to 3-15% of annual oceanic CaCO3 production, yet little is known about how the reaction itself is regulated. Here, the identification and characterization of a proteinaceous organic matrix associated with fish-derived intestinal precipitates will be described. This matrix is capable of regulating CaCO3 precipitation in vitro, an effect which is conserved across matrix derived from several marine fish species. A shotgun proteomic investigation of the Gulf toadfish (Opsanus beta) intestinal response to hypersalinit